What the study found
The study found that a reverse-phase high-performance liquid chromatography (RP-HPLC) method could be developed and validated to measure caffeine, eugenol, and zingerone together in a marketed Ashwagandha Green Tea formulation. The authors describe the method as simple, precise, specific, and robust.
Why the authors say this matters
The authors conclude that the method is suitable for standardising these three phytoconstituents, meaning plant-derived chemical compounds, in a commercial formulation. They also state that design of experiments was applied to robustness, which the study presents as part of integrating experiment-planning methods with RP-HPLC.
What the researchers tested
The researchers developed and validated an RP-HPLC method using a C-18 column and a methanol-water mobile phase in a 35:65 volume ratio. Detection and quantification were carried out at 254 nm for caffeine, eugenol, and zingerone, and the method was validated according to ICH guidelines.
What worked and what didn't
The reported retention times were 4.76 minutes for caffeine, 8.64 minutes for eugenol, and 10.48 minutes for zingerone. The method was validated for linearity, precision, specificity, limit of detection, limit of quantification, accuracy, and robustness, and the calibration plots showed a satisfactory linear relationship in the tested ranges. No failed measurements or negative results are described in the abstract.
What to keep in mind
The summary provided is limited to the abstract, so only the authors' reported validation outcomes are available here. The abstract does not give detailed numerical validation values, full experimental conditions, or limitations beyond the scope of the marketed formulation studied.
Key points
- A validated RP-HPLC method was developed for simultaneous estimation of caffeine, eugenol, and zingerone.
- The method was applied to a marketed Ashwagandha Green Tea formulation.
- Detection and quantification were done at 254 nm using a C-18 column and methanol-water mobile phase.
- Reported retention times were 4.76, 8.64, and 10.48 minutes for caffeine, eugenol, and zingerone.
- The abstract says the method was simple, precise, specific, and robust.
Disclosure
- Research title:
- HPLC method measures caffeine, eugenol, and zingerone in green tea
- Authors:
- Vrutika Patel, Tanvi Dodiya, Disha Prajapati
- Institutions:
- Parul University, Parul University, Parul University
- Publication date:
- 2026-02-26
- OpenAlex record:
- View
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