What the study found
The study found that an RP-HPLC (reversed-phase high-performance liquid chromatography) method could detect and quantify metoprolol and enalapril maleate in bulk and pharmaceutical products. The authors also report that the method worked even when degradants were present.
Why the authors say this matters
The authors say the work supports sustainable alternatives to toxic chemicals and helps reduce waste. They also frame the method as relevant for co-administering the two drugs in a single formulation, which the study notes is important for blood pressure control and cardiovascular risk reduction.
What the researchers tested
The researchers developed an isocratic RP-HPLC method using an InertSustain C8 column, a potassium phosphate buffer at pH 2.5 mixed 50:50 with ethanol, a 5°C sample temperature, and a 40°C column oven. They tested the method with a seven-minute runtime, a 20 µL injection volume, and a 1.0 mL/min flow rate.
What worked and what didn't
The method showed linearity for both metoprolol and enalapril maleate across 3–60 µg/mL, with R2 ≥ 0.999. The abstract reports detection and quantification values for enalapril maleate of 0.29 µg/mL and 0.90 µg/mL, and for metoprolol of 0.33 µg/mL and 0.98 µg/mL. Recovery studies were 98–102%, and the method met International Council for Harmonization criteria; no specific failures are described in the abstract.
What to keep in mind
The abstract does not describe detailed study limitations. It also does not provide full information on the degradants tested or how the method performed relative to other existing methods.
Key points
- The study developed an RP-HPLC method for metoprolol and enalapril maleate.
- The method was reported to work in bulk and pharmaceutical products.
- Linearity was observed from 3–60 µg/mL for both drugs, with R2 ≥ 0.999.
- Recovery studies were 98–102%, suggesting accurate measurement.
- The method followed International Council for Harmonization criteria and handled degradants.
Disclosure
- Research title:
- RP-HPLC quantified metoprolol and enalapril maleate with good accuracy
- Authors:
- Ghadah M. Al-Senani, Salhah D. Al-Qahtani, Samar M. Mahgoub, Aya Shaban, Hoda A. Ahmed, Mahmoud A. Mohamed
- Institutions:
- Beni-Suef University, Cairo University, Cairo University, Hikma Pharmaceuticals (United Kingdom), Princess Nourah bint Abdulrahman University, Princess Nourah bint Abdulrahman University, Taibah University
- Publication date:
- 2026-03-31
- OpenAlex record:
- View
Get the weekly research newsletter
Stay current with scholarly research without reading academic papers — one filtered digest, every Friday.